PCR (Polymer Chain Reaction)
upscales minute concentrations of polynucletides to detectable
amounts. The fragments then are separated by electrophoresis with
respect to chainlength.
Scanned frationation patterns are highly superresolvable, since the kernel is a somooth and perfectly reproducible parameter, especially with modern microtechniques.
The table describes the process of digital superresolution of PCR-data:
Grey solid: Superresolution
Green line: Kernel
|Perform the separation and scan
Modern markers allow for exact mass-propotional detection of polynucleotide patterns, just like in classical HPLC.
Import of the scanned data into a resolver software is all, that's needed to achieve approximate duplicate resolution and removal of peak tails and asymmetries.
The example shows, how seemingly 8 peaks decompose into twelve ones.
Calculation time is less than 5 seconds and can be fully automatted.
|Reducing sampling intervals,
High throughput optimization
|Please consult the page 'Reducing sampling times in chromatography''|
requires robust baseline correction.
In PROANALYSI::PEAKS, you find baseline-routines optimized especially for PCR.
The complete sequence of data analysis can be automatted and perfomed by simply pressing a button.